integration n. 1.结合;综合;一体化。 2.【心理学】整合(作用)。 3.【数学】积分(法) (opp. differentiation)。 4.【经济学】(产业的)集中。 5.〔美国〕取消种族隔离;给以种族上的平等待遇。 integration by parts 【数学】部分积分法。
site n. 1.地点;位置;地基。 2.场所,现场。 3.遗址。 4.【计算机】网站,站点〔电脑网络用户的网站地址〕;万维网址(=Website)。 construction site 建筑工地。 firing [launching] site (火箭等)发射场。 historic sites 历史遗址。 nuclear test site 核试验场。 the site of a battle 战场。 vt. 给与位置,为…选定地点;安放;使坐落于(某处)。 adj. -ed 地点[位置]…的 (a well-sited factory 地点好的工厂)。
The mechanisms of gene silencing include copy numbers and configuration of transgene , integration sites in plant and transcription of transgene , etc 基因沉默的机制是多方面的,包括转基因的拷贝数和构型、在植物上的整合位点、转基因的转录水平等。
To abolish transcription efficiency affected by copy number or integration site in chromosome , all the vectors were integrated into frt site by co - transfecting with an flp recombinase producing plasmid 应用w七stemblot对其中的20个克隆进行检测,选出一个caspase一3表达量最低的细胞克隆a6 。
In abroad , the study of integration site used for transgenic detection had just begun . in this study , according to the collection of the global commercialized transgenic crops , select seven exogenous genes which basically cover the total commercialized crops , namely camv35s and fmv promoter , nos terminater , mark gene nptii , and aim genes pat , epsps and cryia ( b ) . use endogenous 18srrna gene as collate , design a large pairs of specific primers , screen the optimum primers groups , optimized the test condition and parameters , establishing the qualitative pcr detection system 本研究根据收集的国内外已商品化的转基因作物品种,选择了能基本覆盖商品化转基因品种的7个外源基因,即: camv35s 、 fmv启动子、 nos终止子、 npt标记基因和目的基因pat 、 epsps 、 cryia ( b )作为筛选目标,以植物18srrna基因作为内源参照基因,设计了多对特异性引物,并筛选出最佳组合,优化了检测条件和参数,建立了pcr定性检测方法体系。
Transgenic integration site detection is the efficient method to identify whether the transgenic crops are approved to import or export . some quantitative detection methods were reported in 2000 and 2001 , and that is about bt11 and mon810 corn . real - time 5 " - nuclease pcr had been previously used successfully to determine quantitatively roundup ready ( rr ) soy and btl76 maize in food 转基因整合位点检测是鉴定转基因品种是否为批准进口转基因作物的有效的特异性方法,国外在2000年和2001年已开展了转基因玉米bt11 、 mon810两个品种整合位点的特异性检测方法研究。
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element , containing receptor binding sites and major antigenic determinants . exjsrv - specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals , validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences 用地高辛随机引物法标记exjsrv特异的env片段,制备探针,原位杂交检测spa肺组织中的rna及前病毒dna ,结果表明spa患羊肺组织内有jsrvenv基因mrna的表达,同时也检测到了前病毒dna ,而相应的阴性对照却无阳性信号,证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。
Though the technique of nuclejc transformation in plants has been developed and used widely , some problems in genetic information have not been resolved . for example , because the nucleic genome is so big and complicated that the integration sites and copies of foreign gene can not be controlled accurately , the expression of transferred genes is inefficient as a result of gene silencing or position effect . in nucleic transformation , furthermore , the transfer of multigene is difficult , and only after the prokaryotic genes undergo modification are they expressed in high plants 植物的细胞核转化技术已发展成熟并得到广泛应用,但核基因组的遗传转化仍存在一系列至今尚未解决的问题:例如由于核基因组大,背景复杂,外源基因的整合位点和整合的拷贝数难以人为控制,造成郑州大学2003年博士学位论文杜氏盐藻( dunaliellasalina )叶绿体转化研究外源基因表达效率低,容易出现基因失活、基因沉默、位置效应等现象;同时转入多个基因时操作步骤过于复杂,所表达的原核基因必须经过修饰改造,环境安全难以保证等。